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Assessment of various seed-borne fungi association in onion (Allium cepa L.) seeds by blotter paper and PDA method of isolation and detection


The International Journal of Biological Research (TIJOBR)

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Hafiza Hadiqa Anum1*, Muhammad Hadi Abbas1, Muhammad Ali Ahsen1, Mirza Waqas Safder2, Sami Mohsin1, Hasham Ali1, Shahid Iqbal2, Muhammad Muneeb Shabbir3 and Muhammad Tariq4

-Corresponding author: hadiqaanum120@gmail.com  

Submitted Accepted Published
Apr 22,2018 Nov 13,2018 Dec 20,2018

2018 / Vol: 1 / Issue: 1


Abstract


Onion is one of the most important commercial vegetable crops grown in Pakistan. It is valued for its distinct pungent flavor and is an essential ingredient for the cooking in many regions. Seed born fungus on onion seed cause seed.abortion, seed.rot, sclerotisation or stromatisation of seed, seed.necrosis, decrease or removal of germ inability and sprout injury which resulting in the growth of ailment in later-stages of plant development by universal or native poison. In this study, seed born fungus was detected from infected onion seeds by using blotter paper method and agar plate method. Seed born fungus was detected by using different fungus detecting methods, consequence of seed born fungus on seed sprouting. The results obtained that the maximum incidence of A. porri (4%), A. niger (2.29%) and A. flavus (1.50%) while minimum incidence of F. oxysporum (0.99%) and Botrytis spp. (0.67%) were recorded.

Key words: Fungus, Blotter, PDA, Incidence



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