• The International Journal of Biological Research (TIJOBR)- Published Quarterly
  • The International Journal of Global Sciences (TIJOGS) -Published Quarterly

Confirmation of ALS inhibitor herbicide resistance in Echinochloa colonum

The International Journal of Biological Research (TIJOBR)


Faisal Mushtaq1*, Abdur Rehman1, Shumila Shahid2, Hafiz Muhammad Umair Waqas4, Hafiz Muhammad Waseem1, Saeed Ahmad3, Sobia Manzoorand Zeeshan Latif1

Corresponding author: faisalmushtaq3767@gmail.com
Submitted Accepted Published
Jul 18,2018 Dec 11,2018 Jan 13,2019

2019 / Vol: 2 / Issue: 1


Herbicides resistance is emerging as an important trend in global farming and this problem is increasing significantly. Herbicide resistance is developed in plants with the passage of time through evolution. ALS (Acetolactate synthase) is enzyme naturally occurred in plants involves in synthesis of amino acid and involved to catalyze the initial process of synthesis of various proteins that are branched in character. Bispybribic sodium herbicide inhibits the protein synthesis and generally marked as vital herbicide against weeds infestation in rice. The present experiment was performed to evaluate the prospective of ALS inhibitor herbicides resistance on different accessions of Jungle rice (Echinochloa colonum L.) under CRD with factorial arrangement. Mortality %, Visual injury %, LD50, GR50, Resistance index and Biomass % decrease over control parameters were recorded features of proposed experiment. The date of recorded parameters were analyzed properly by applying Fisher’s analysis of variance method and average data of all treatments were associated by least significant difference (LSD) test having 5% level of probability. After the application of treatments, the plants were supplied adequate amounts of water and nutrients for 30 days. Plant death (live or dead counts) was recorded 30 d after treatment (DAT). The application of four different doses of the herbicide was performed after 15 days of germination, with seedlings at the stage of 2 to 3 leaves. Sprayer configuration and growth condition for all biotypes for same. Significant and high biomass was showed by ECFS-7 (76.5) and ECFS-4 (75.66) as compared to EC-FS-SS (31.007). Less injury rate was showed by ECFS-4 (50.12) and ECFS-7 (53.69%) as compared to EC-FS-SS which show maximum injury percentage (72.18%). Maximum mortality percentage was showed in EC-FS-SS (76.062) and less in EC-FS-7 (42.37 %).   LD50 of Accessions at different levels was analyzed with PROC PROBIT. The highest LD50 values were observed in EC-FS-7 (1.60) which was followed by EC-FS-5. The lowest value of LD50 was observed in susceptible biotype. The EC-FS-7 shows maximum GR50 as compared to EC-FS-SS. The maximum growth reduction was recorded at double dose in all accessions. The EC-FS-7 showed maximum RI value as compared to EC-FS-SS. The lowest resistance index is recorded in susceptible EC-FS-SS. The lowest value of susceptible standards indicates that lower dose of herbicide is required to kill the weeds due to low resistance against herbicide.

Key words: ALS inhibitor herbicides, resistance, Jungle rice (Echinochloa colonum L.)


  1. Akbar, N., Ehsanullah, K. Jabran and M. A. Ali. 2011. Weed management improves yield and quality of direct seeded rice. Aust. J. Crop Sci. 5: 688–694.
  2. Baltazar, A. M. (2017), Herbicide resistant weeds in the Philippines: Status and resistance mechanisms. Weed Biol. Manag., 17: 57-67.
  3. Beckie, H. J. and Harker, K. N. (2017). Our top 10 herbicide‐resistant weed management practices. Pest.Manag. Sci, 73: 1045-1052.
  4. Bhushan, L., J. K. Ladha, R. K. Gupta, S. Singh, A. Tirol-Padre and Y. S. Saharawat. 2007. Saving of water and labor in a rice–wheat system with no-tillage and direct seeding technologies. Agron. J. 99: 1288–1296.
  5. Dobermann, A., and T. Fairhurst. 2000. Rice: Nutrient Management and Nutrient Disorders. PPI/PPIC and IRRI, 162 p.
  6. García-Garijo, A., Palma, F., Iribarne, C., Lluch, C., and Tejera, N. A. (2012). Alterations induced by imazamox on acetohydroxyacid synthase activity of common bean (Phaseolus vulgaris) depend on leaf position. Pesticide Biochemistry and Physiology, 104: 72-76.
  7. Gerwick, B. C., Subramanian, M. V., Loney-Gallant, V. I., and Chandler, D. P. (1990). Mechanism of action of the 1,2,4-triazolo[1,5-a]pyrimidines. Pesticide Science, 29: 357-364. 
  8. Guttieri, M. J., Vberlein, C. V., and Thill, D. C. (1995). Diverse mutations in the actolactate synthase gene confer chlorsulfuron resistance in Kochia (Kochiascoparia) biotypes. Weed Science, 43: 175-178.
  9. Heap, I. (2013), Global perspective of herbicide‐resistant weeds. Pest.Manag. Sci., 70: 1306-1315.
  10. Hilu, Khidir W. "Evolution of finger millet: evidence from random amplified polymorphic DNA." Genome 38, no. 2 (1995): 232-238.
  11. Islam, M.S., S. Peng, R.M. Visperas, M. Sultan, S. M. Altaf and A.W. Julfiquar. 2010. Comparative study on yield and yield attributes of hybrid, inbred and NPT rice genotypes in a tropical irrigated ecosystem. Bangladesh J. Agric. Res. 35:343-353.
  12. Jabran, K., Ehsanullah, M. Hussain, M. Farooq, M. Babar, M. N. Dog and D. J. Lee. 2012. Application of bispyribac-sodium provides effectiveweed control in direct-planted rice on a sandyloam soil. Weed Bio. Manage. 12: 136–145.
  13. Kumar, V.A., Mohan, T.K. and Murugan, K., 2008. Purification and kinetic characterization of polyphenol oxidase from Barbados cherry (Malpighia glabra L.). Food Chemistry,  110(2), pp.328-333.
  14. Powles, S. B., and Holtum, J. A. (1996). Herbicide resistance in plants: biology and biochemistry. Boca Raton: Lewis Publishers Inc.
  15. Rao, A.N., Johnson, D.E., Sivaprasad, B., Ladha J.K. and A. M. Mortimer, A.M. (2007). Weed management in direct-seeded rice. Advan.Agron.93:153–255.
  16. Rao, A.N., Johnson, D.E., Sivaprasad, B., Ladha J.K. and A. M. Mortimer, A.M. (2007). Weed management in direct-seeded rice. Advan.Agron.93:153–255.
  17. Ray, T. B. (1984). Site of action of chlorsulfuron-      inhibition of valine and isoleucine biosynthesis in plants. Plant Physiology, 75: 827-831.
  18. Razzaq A., Z.A Cheema, K. Jabran, M. Hussain, M. Farooq and M. Zafar.2012. Reduced herbicide doses used together with allelopathic sorghum and sunflower water extracts for weed control in wheat. J. Plant Prot. Res. 52: 281–285.
  19. Shaner, D. L., Anderson, P. C., and Stidham, M. A. (1984). Potent inhibitors of acetohydroxyacid synthase. Plant Physiology, 76: 545-546.
  20. Singh, A.K., B.U. Choudhury and B.A.M. Bouman. 2003. Effects of rice establishment methods on crop performance, water use and mineral nitrogen. In: Water-Wise Rice Prodduction. pp. 223-235. Proceedings of a Thematic Workshop on Water-Wise Rice Production, 8-10 April, 2002 at IRRI Headquarters in Los Banos, Philippines. Int. Rice Res. Institute, Los Banos, Philippines.
  21. Takahashi, S., Shigematsu, S., Morita, A., Nezu, M., Claus, J. S., and Williams, C. S. (1991).KIH-2031, a new herbicide for cotton. Brighton Crop Protection Conference (pp. 57-62). Farnham, U.K.: Brighton Crop Protection Council.
  22. Tan, S., Evans, R. R., Dahmer, M. L., Singh, B. K., and Shaner, D. L. (2005). Imidazolinone-tolerant crops: history, current status, and future. Pest Management Science, 61: 246-257.
  23. Tranel, J. R. and Wright, R. T. (2002). Resistance of Weeds to ALS-Inhibiting Herbicides: What Have We Learned? Weed Science Vol. 50, No. 6 (Nov. - Dec., 2002), pp. 700-712.
  24. Wright, T. R., and Penner, D. (1998). Corn (Zea mays) acetolate synthase sensitivity to four classes of ALS-inhibiting herbicides. Weed Science, 46: 8-12.
  25. Wu, C., Davis, A. S. and Tranel, P. J. (2018), Limited fitness costs of herbicide‐resistance traits in Amaranthustuberculatus facilitate resistance evolution. Pest.Manag. Sci, 74: 293-301

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