• The International Journal of Biological Research (TIJOBR)- Published Quarterly
  • The International Journal of Global Sciences (TIJOGS) -Published Quarterly

Detection and Identification of Fungi Associated with onion (Allium cepa L.) Seeds and their management through Bio Agents and Chemicals

The International Journal of Biological Research (TIJOBR)


Bismillah Khan1, Mustansar Mehmood2, Aaliya Batool1, Zoya Batool Naqvi1, M. Anas Bahoo3, Rida Hayat1

Urwa tul Wusqa1 , Umar Muaz2

1Department of Botany, University of Agriculture, Faisalabad, Pakistan.

2Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan.

3Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.

*Corresponding author’s email: bismillahk823@gmail.com 

Submitted Accepted Published
May 11,2019 Jul 23,2019 Jul 30,2019

2019 / Vol: 2 / Issue: 2


Onion is one of the most important commercial vegetable crops grown in Pakistan. It is valued for its distinct pungent flavor and is an essential ingredient for the cooking in many regions. In this study, seed born fungus was detected from infected onion seeds by using blotter paper method and agar plate method. Seed born fungus was detected by using different fungus detecting methods, consequence of seed born fungus on seed sprouting and integrated management of seed born mycoflora by different chemicals and plant extracts. During the evaluation of detection methods, the fungus incidence was observed at higher level in PDA method as compared to Blotter paper method. Seed treated with Hombre gave maximum seed germination and minimum recovery of fungi as compared to control germination and other treated seeds. While the seeds sown without treatment of any fungicide suspension showed the lowest germination. In study onion seeds were also treated with different plant extracts e.g. garlic, neem and Safeda. The maximum seed germination was observed in case of neem treated seeds. Other plant extract garlic and Safeda showed intermediate growth.

Keys words: Onion, seed borne fungus, plant extracts


  1. Akgul, D.S., H. Ozgonen and A.  Erkilic, 2011. The effects of seed treatments with fungicides on stem rot caused by sclerotium rolfsii sacc., in peanut. Pak. J. Botnay, 43:2991-2996.
  2. Alberto, R.T., 2014. Pathological response and biochemical changes in Allium cepa L. infected with anthracnose twitter disease. Plant Pathology Quarantine. 4:23-31. 
  3. Boff, P., M.J. Standik, R. Ferrari, T.D. Silva, 1995. Assessment of health status of onion seed commercialized in the state Santa Catarina. Revista Brasileira de sementes. 17:165-170. 
  4. Cramer, C.S., 2000. Breeding and genetics of Fusarium basal rot resistance in onion. Euphytica., 115: 159-166. 
  5. Fakir, G. A. 1998. An annotated list of seed borne diseases in Bangladesh. Bangladesh Agric. Univ. Mymensingh, 25 p. 
  6. FAO., 2011. Food and Agriculture Organization. (http://faostat.org/faost at/servlet). Accessed on 3rd January 2012. 
  7. Geremew, A., A. Teshome, T. Kasaye and C. Amenti, 2010. Effect of intrarow spacing on yield of three onion (Allium cepa l.) varieties at Adami Tulu agricultural research center (mid rift valley of Ethiopia). J. Hortic. For.  2:007-011, January, 2010. 
  8. Govindachari, T.R., G., Suresh, G., Gopalakrishnan, B. Banumathy, and S. Masilamani, 1998. Identification of antifungal compounds from the seed oil of Azadirachta Indica. Phytoparasitica. 26:109-116. 
  9. Gupta, R. and V. Pathak, 1988: Yield losses in onions due to purple blotch disease caused by Alternaria porri. – Phytophylactica 20:21–23. 
  10. Gupta, R., M.K.  Khokhar and R.A.M.  Lal, 2012. Management of the black mould disease of onion. J. of plant Pathol. and Microbiol., 3:1-3. 
  11. Gupta, R.P. and V. Mehra, 1984. Studies on the effect of fungal metabolites on viability and seedling vigour of onion seed. Indian J. of Plant Pathol., 2:100 – 102.
  12. Gupta, R.P. and V. Mehra, 1984. Studies on the effect of fungal metabolites on viability and seedling vigour of onion seed. Indian J. of Plant Pathol., 2:100 – 102. 
  13. Hanelt, P. 1990. "Taxonomy, Evolution, and History." In Onions and Allied Crops, edited by Haim D. Rabinowitch and James L. Brewster, 1-26. Boca Raton, Fla.: CRC Press,
  14. Hussein, M., M. Hassan, A. Allam, K. Abo-Elyousr, 2007: Management of Stemphylium blight of onion by using biological agents and resistance inducers. – Egyptian J. of Phytopathol. 35:49–60. 
  15. Kantona, R.A.L., L. Abbeyb, R.G. Hillac, M.A. Tabil and N.D. Jane, 2003. Density affects plant development and yield of bulb onion (Allium cepa L.) in Northern Ghana. J. Veg. Crop Prod. 8:15-25. 
  16. Ko, S., J. Huang J. Wang, S. Subramanyam and W. Chang, 2002. Evaluation of onion cultivars for resistance to Aspergillus niger, the causal agent of black mold. J. of American Society of Hort. Sci., 127:697-702. 
  17. Köycü, N.D. and N. Ozer, 1997. Determination of seed-borne fungi of onion and their transmission to onion seeds. Phytoparasitica, 25:25-31. 
  18. Kumar, U., P. Naresh and S.K. Biswas, 2012. Eco-friendly management of stem phylium blight (Stemphylium botryosum) of garlic by plant extract and bio-agents. Hortflora Res. Spect. 1:42-45 
  19. Mathur Kamlesh, R.B.S., Gurjar S.N. Shanna, and K. Sharma, 2007. Efficacy of fungicides, bioagents and plant extracts against pink root rot disease of onion induced by Fusarium solani. J. of Mycol. and Plant Pathol., 37(3). 
  20. Neergaard, P., 1979. Seed Pathology. Vol 1. The Macmillan Press, London. 
  21. Ozer, N. and N.D. Koycu, 1997. The pathogenicity of Aspergillus niger and some Fusarium species on onion seed and seedlings. In: Proceedings of the 10th Congress of the Mediterranean Phytopathological Union, 1-5 June 1997, Monpellier (France), pp. 277-281. 
  22. Rajam, S.R., 1992. Studies on the post-harvest fungal spoilage of onion. M.Sc. Ag.) Thesis (Unpublished), Tamil Nadu Agric. Univ., Coimbatore. 
  23. Rehman, G.M., M.R. Islam and M.A. Wadud, 1999. Seed treatment with plant extracts and hot water, a potential biophysical method of controlling seed borne infection of wheat. Bangladesh J. Train. Develop. 12:185-190.
  24. Schwartz, H., 2004: Botrytis, downymildew and purple blotch of onion. Colorado State Univ. Cooperative Extension No. 2.941. 
  25. Shehu, K. and S. Muhammad, 2011. Fungi associated with storage rots of onion bulbs in Sokoto. Nigeria. Int. J. of Mod. Biol., 1:1-3. 
  26. Sinclair, P.J. and D.B.  Letham, 1996. Incidence and sites of visible infection of Aspergillus niger on bulbs of two onion (Allium cepa) cultivars. Australian Plant Pathol., 25:811. 
  27. Solanke, R.B., D.B. Deosarkar and L.N. Jawale, 2001. Seed born fungi of chilli and response of fusarium moniliformi  to various seed dressers . J. Maharashtra agric. Univ., 26:187-188. 
  28. Utikar, P. and D. Padule, 1980: A virulent species of Alternaria causing leaf blight of onion. – Indian Phytopathology 33:335–336. 
  29. Uzeh, R.E., F.A. Alade and M. Bankole, 2009. The Microbial Quality of Pre-packed Mixed Vegetable Salad in Some Retail outlets of Lagos, Nigeria. African J. of Food sci. 3:270-272. 
  30. Wani, A.H. and T.U. Nisa, 2011. Management of black mold rot of onion. Mycopath. 9:43-49.   
  31. Wani, A.H. and Taskeen, 2011. Management of black mold rot of onion. Mycopathol., 9:43-49


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