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Detection and identification of seed associated fungi of sesame (Sesamum indicum) during storage and their management


The International Journal of Biological Research (TIJOBR)

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Zulnoon Haider1, Rafia Asghar2, Sadia Nazir3, Maham Mustafa1, Azra Razzaq1, Nazish Raza1, Faisal Imran4

1Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan.

2Department of Plant Pathology, Faculty of Food Crop & Sciences, PMAS Aid Agriculture University

3Department of BotanyUniversity of Agriculture, Faisalabad, Pakistan.

4Institute of Agricultural Extension and Rural Development, University of Agriculture, Faisalabad, Pakistan.

*Corresponding email: zulnoonhaider51214@gmail.com
Submitted Accepted Published
Oct 22,2019 Dec 28,2019 Jan 29,2020

2020 / Vol: 3 / Issue: 2


Abstract


Sesame (Sesamum indicum L.) is one of the most ancient oilseed crop and rich source of oil, protein, calcium as well as phosphorus, which is cultivated throughout Pakistan. Different pathogens affect the sesame crop and causing diseases including some post-harvest and storage fungi causing serious losses in production. These seeds borne fungi causes heavy damage by decreasing seed germination. Infected germinated seeds do not grow properly which increased the chance of seedling concision and pre or post emergence diseases consequences into inhibited growth and yield losses. By considering the above evidences the current research is intended to determine the seed borne fungi related with sesame seeds and their management. For this purpose seed samples were collected from seed market of Faisalabad and Ayub Agriculture Research Institute (AARI) (250 g/sample). These seed samples were platted on blotter paper and agar plates. These plates were incubated at 25±2ºC and number of fungi were observed and identified with fungal colony color, sporulation type and relevant literature after 6-8 days of incubation. During management trials the evaluation of treated and non-treated seeds were done by using different seed dressing fungicides and plant extracts. These research trials were carried out under Complete Randomized Design (CRD) and the statistical significance between mean values were recorded using LSD test. During the detection experiments, Alternaria alternata, Fusarium moniliforme, F. oxysporum, A. tenuis, S. rolfsi, Cercospora sesami, Curvularia lunata, Macrophomina phaseolina, Aspergillus niger, A. flavus, A. ochraceus, A. versicolor, A. terreus, A. candidus, Haplosporangium sp., Penicillium citratum, Rhizopus nigricans, and R, stolonifer were isolated from local variety of sesame seeds. Among all the seed health test methods, standard blotter method is most superior for detection of seedborne fungi over the other methods. 

Key words: Sesame, seed associated fungi, seed health test methods


Reference


1.Elewa, I. S. , Mostafa, M. H. , Sahab, A. F. and Ziedan, E. H.(2011) 'Direct effect of biocontrol agents onwilt and root-rot diseases of sesame', Archives Of Phytopathology And Plant Protection, 44: 5, 493 — 504 

2.Hammad Nawaf Farhan, Basher H. Abdullah & Ashwaq T. Hameed. 2010. The biological activity of bacterial vaccine of Pseudomonas putida2 and Pseudomonas fluorescens3 isolates to protect sesame crop (Sesamum indicum) from Fusarium fungi under field conditions. Agric. Biol. J. N. Am., 2010, 1(5): 803-811.

3.ISTA. 1999. International rules for seed testing. Prof. Inst. Seed test. Assoc., 31: 1-152

4.Jonsyn Felixtina E. 1988. Seedborne fungi of sesame (Sesamum indicum L) in Sierra Leone and their potential aflatoxin/mycotoxin production. Mycopathologia 104:123-127 

5.Khokhar, I., Haider, M. S., Mukhtar, I., Ali, A., Mushtaq, S. and Ashfaq, M. (2013). Effect of Penicillium species culture filtrate on seedling growth of wheat. 3(1) pp. 24-29.

6.Richardson MJ (1990). An annoted list of seed-borne disease (4th edition). Proceeding of the International Seed Testing Association Zurich, Switzerland.

7.Sahab AF, Elewa IS, Mostafa MH, Ziedan EH. 2008. Morphological, physiological andpathological investigation on Fusarium oxysporumf.sp.sesami. Egypt J Agric Res. NRC.1(2):167–181 


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