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Detection of Bla (Bic) Bla (Gim) Tem-116 Metallo Beta Lactamases Gene In Pseudomonas Aeruginosa From Cancer Patients


The International Journal of Biological Research (TIJOBR)

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Nida andlib1, Muhammad Farhan1*, Basit Zeeshan1, Summiyan Nizamuddin2

1Faculty of Life Science, University of Central Punjab Lahore

2Shaukat Khanum Memorial Cancer Hospital& Research Center

*Corresponding author: farhanlatif6242@gmail.com 

Submitted Accepted Published
Jan 05,2020 Mar 22,2020 Mar 26,2020

2020 / Vol: 3 / Issue: 2


Abstract


The study was conducted for the detection of beta lactamase genes which includes TEM-116, bla GIM and bla BIC in multi drug resistant Pseudomonas aeruginosa from cancer patients. These genes play the most significant role in multi drug resistance. In this study, we used the disc diffusion method for the determination of antibiotic resistance against seven antibiotics and PCR technique for the detection of genes. The results showed that the P. aeruginosa isolates were resistant against Sulfamethoxazole/Trimethoprim (SXT) 84%, Ciprofloxacin (CIP) 48%, and Ceftozane/Tezobactum (CT) 76%, Cefipime (FEP) 80%, Ceftazidime (CAZ) 36%, Amikacin (AK) 30%, Levofloxacin (LEV) 28%. The PCR results showed that 46 (92%) isolates were positive for the TEM-116 and no isolate was positive for BIC and GIM genes. These results revealed high emergence of resistance in TEM-116 of P. aeruginosa isolates studied. This is the first report showing detection of TEM-116 resistant gene against P. aeruginosa in cancer patient from Pakistan. The study might be helpful in understanding the molecular basis of antibiotic resistance in P. aeruginosa from cancer patients.

Key words: METALLO BETA LACTAMASES GENE, PSEUDOMONAS AERUGINOSA, CANCER


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