• The International Journal of Biological Research (TIJOBR)- Published Quarterly
  • The International Journal of Global Sciences (TIJOGS) -Published Quarterly

Detection of Bla (Bic) Bla (Gim) Tem-116 Metallo Beta Lactamases Gene In Pseudomonas Aeruginosa From Cancer Patients

The International Journal of Biological Research (TIJOBR)


Nida andlib1, Muhammad Farhan1*, Basit Zeeshan1, Summiyan Nizamuddin2

1Faculty of Life Science, University of Central Punjab Lahore

2Shaukat Khanum Memorial Cancer Hospital& Research Center

*Corresponding author: farhanlatif6242@gmail.com 

Submitted Accepted Published
Jan 05,2020 Mar 22,2020 Mar 26,2020

2020 / Vol: 3 / Issue: 2


The study was conducted for the detection of beta lactamase genes which includes TEM-116, bla GIM and bla BIC in multi drug resistant Pseudomonas aeruginosa from cancer patients. These genes play the most significant role in multi drug resistance. In this study, we used the disc diffusion method for the determination of antibiotic resistance against seven antibiotics and PCR technique for the detection of genes. The results showed that the P. aeruginosa isolates were resistant against Sulfamethoxazole/Trimethoprim (SXT) 84%, Ciprofloxacin (CIP) 48%, and Ceftozane/Tezobactum (CT) 76%, Cefipime (FEP) 80%, Ceftazidime (CAZ) 36%, Amikacin (AK) 30%, Levofloxacin (LEV) 28%. The PCR results showed that 46 (92%) isolates were positive for the TEM-116 and no isolate was positive for BIC and GIM genes. These results revealed high emergence of resistance in TEM-116 of P. aeruginosa isolates studied. This is the first report showing detection of TEM-116 resistant gene against P. aeruginosa in cancer patient from Pakistan. The study might be helpful in understanding the molecular basis of antibiotic resistance in P. aeruginosa from cancer patients.



1)Ahmad, M., M.  Hassan, A. Khalid, I. Tariq, M.H.H.B. Asad, A. Samad, and G. Murtaza. 2016. Prevalence of Extended Spectrum β-Lactamase and Antimicrobial Susceptibility Pattern of Clinical Isolates of Pseudomonas from Patients of Khyber Pakhtunkhwa, Pakistan. Bio Med Research International.

  1. Alipour, T., N. Sadeghifard, N.Amirmozafari, S.Ghafurian, A.S. Abdulamir, R. Mohebi, and M. Raftari.2010. Incidence of extended spectrum β-lactamases producing Pseudomonas aeruginosa and frequency of OXA-2 and OXA-10 genes. Australian Journal of Basic and Applied Sciences. 48:3202-7.
  2. Bashir, D., M.A. Thokar, B.A. Fomda, G. Bashir, D. Zahoor, S.Ahmad, and A.S. Toboli. 2011. Detection of metallo-beta-lactamases (MBL) producing Pseudomonas aeruginosa at a tertiary care hospital in Kashmir. African Journal of Microbiology Research. 52:164-172.
  3. Bonomo, R. A., and D. Szabo. 2006. Mechanisms of multidrug resistance in Acenitobacter species and Pseudomonas aeruginosa. Clinical Infectious Diseases. 49:S49-S56.
  4. Charrakh, A. H., S.J. Al-Awadi, and A.S. Mohammed.2016. Detection of Metallo-β-Lactamase Producing Pseudomonas aeruginosa Isolated from Public and Private Hospitals in Baghdad, Iraq. ActaMedicaIranica. 542:107-113.
  5. Dumas, J. L., C. Van Delden, K. Perron, and T. Kohler. 2006. Analysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real-time-PCR. FEMS Microbiology Letters. 2542:217-225.
  6. Faghri, J., M. Pourentezari, M. Esmaeily, S. Pirouzi, and M. Sedighi. 2014. Prevalence of metallo-beta-lactamases genes blaVIM-1 and blaSPM-1 in Pseudomonas aeruginosa Clinical Isolates in Isfahan, Iran. Global Journal of Medicine Researches and Studies. 11:20-27.
  7. Ganju, S. A., R.C. Guleria, S.Bhagra, and A.K. Kanga.2015. Screening for metallo-β-lactamases producing Pseudomonas aeruginosa in clinical isolates in a tertiary care hospital in North India. Medical Journal of Dr. DY Patil University. 83:334.
  8. Ghazawi, A.,  A. Sonnevend,  R.A. Bonnin,   L. Poirel,  P. Nordmann, R. Hashmey,  and T. Pal. 2012. NDM‐2 carbapenamase‐producing Acenitobacter baumannii in the United Arab Emirates. Clinical Microbiology and Infection. 182:34-36.
  9. Hammami, S., I.B.B. Boubaker, R. Ghozzi, M. Saidani, S. Amine, and S.B. Redjeb. 2011. Nosocomial outbreak of Imipenem-resistant Pseudomonas aeruginosa producing VIM-2 metallo-β-lactamases in a kidney transplantation unit. Diagnostic Pathology. 61:106.
  10. Hassuna, N. A., A.H.I. Mohamed, S.M. Abo-Eleuoon, and H.A.W.A. Rizk. 2015. High prevalence of multidrug resistant Pseudomonas aeruginosa recovered from infected burn wounds in children. Archives of Clinical Microbiology.
  11. Khan, F., A. Khan, and S.U. Kazmi.2014. Prevalence and Susceptibility Pattern of Multi Drug Resistant Clinical Isolates of Pseudomonas aeruginosa in Karachi. Pakistan Journal of Medical Sciences. 305:951-954.
  12. Mahmoud, A. B., W.A Zahran, G.R. Hindawi, A.Z. Labib, and R. Galal.2013. Prevalence of multidrug-resistant Pseudomonas aeruginosain patients with nosocomial infections at a University Hospital in Egypt, with special reference to typing methods. Journal of   Virology and Microbiology.13.
  13. Mansour, S. A., O. Eldaly, A. JimanFatani, M.L. Mohamed, and E.M. Ibrahim. 2013. Epidemiological characterization of P. aeruginosa isolates of intensive care units in Egypt and Saudi Arabia.
  14. Peymani, A., T.N. Farivar, R. Sanikhani, A.Javadi, and R. Najafipour. 2014. Emergence of TEM, SHV, and CTX-M-Extended Spectrum β-lactamases and class 1 integron among Enterobacter cloacae isolates collected from hospitals of Tehran and Qazvin, Iran. Microbial Drug Resistance. 205:424-430.
  15. Picao, R. C., L. Poirel, A.C. Gales, and P.Nordmann. 2009. Diversity of β-lactamases produced by ceftazidime-resistant Pseudomonas aeruginosa isolates causing bloodstream infections in Brazil. Antimicrobial Agents and Chemotherapy. 539:3908-3913.
  16. Pitout, J. D., D.B. Gregson, L. Poirel, J.A. McClure, P. Le, and D.L. Church. 2005. Detection of Pseudomonas aeruginosa producing metallo-β-lactamases in a large centralized laboratory. Journal of Clinical Microbiology. 437:3129-3135.
  17. Rossolini, G. M., and E. Mantengoli.2005. Treatment and control of severe infections caused by multi resistant Pseudomonas aeruginosa. Clinical Microbiology and Infection. 114:17-32.
  18. Shah, D. A., S. Wasim, and F.E. Abdullah.2015. Antibiotic resistance pattern of Pseudomonas aeruginosa isolated from urine samples of Urinary Tract Infections patients in Karachi, Pakistan. Pakistan Journal of Medical Sciences. 312:341.
  19. Strateva, T., and D. Yordanov.2009. Pseudomonas aeruginosa a phenomenon of bacterial resistance. Journal of Medical Microbiology.589:1133-1148.
  20. Souli, M., I. Galani, and H. Giamarellou.2008. Emergence of extensively drug-resistant and pan drug-resistant Gram-negative bacilli in Europe. Euro surveillance: bulletin Europeensur les maladies transmissibles= European communicable disease bulletin. 1347:5437-5453.
  21. Teixeira, B., H.Rodulfo, N. Carreno, M. Guzman, E. Salazar, and M.D. Donato. 2016. Aminoglycosides resistance genes in Pseudomonas aeruginosa isolates from Cumana, Venezuela. Revista do Instituto de Medicina Tropical de São Paulo.58.
  22. Toval, F., A. Guzmán-Marte, V.Madriz, T. Somogyi, C. Rodríguez, and F. García.2015. Predominance of carbapenem-resistant Pseudomonas aeruginosa isolates carrying blaIMP and blaVIM metallo-β-lactamases in a major hospital in Costa Rica. Journal of Medical Microbiology. 641:37-43.
  23. Wolter, D. J., N. Khalaf, I.E. Robledo, G.J. Vázquez, M.I. Santé, E.E. Aquino, and N.D. Hanson.2009. Surveillance of carbapenem-resistant Pseudomonas aeruginosa isolates from Puerto Rican medical center hospitals: dissemination of KPC and IMP-18 β-lactamases. Antimicrobial Agents and Chemotherapy. 534:1660-1664.
  24. Yan, J. J., P.R. Hsueh, W.C. Ko, K.T. Luh, S.H. Tsai, H.M. Wu, and J.J. Wu. 2001. Metallo-β-Lactamases in Clinical Pseudomonas Isolates in Taiwan and Identification of VIM-3, a Novel Variant of the VIM-2 Enzyme. Antimicrobial Agents and Chemotherapy. 458:2224-2228.
  25. Zhang, L., M. Fritsch, L. Hammond, R. Landreville, C. Slatculescu, A. Colavita, and T.F. Mah. 2013. Identification of genes involved in Pseudomonas aeruginosa biofilm-specific resistance to antibiotics. PLoS One. 84:616-25.

© Copy Rights
By Authors and RnD Journals.