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DETECTION OF CARBAPENEM RESISTANT METALLO-BETA LACTAMASES GENE AMONG PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM CANCER PATIENTS


The International Journal of Biological Research (TIJOBR)

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Muhammad Farhan1*, Nida andlib1, Javed Iqbal Wattoo1, Basit Zeeshan1

1Faculty of Life Science, University of Central Punjab Lahore

*Corresponding author: farhanlatif6242@gmail.com 

Submitted Accepted Published
Jan 24,2020 Mar 15,2020 Mar 26,2020

2020 / Vol: 3 / Issue: 1


Abstract


The study was conducted for the detection of carbapenem resistant beta lactamase genes including VIM, and IMP in multi drug resistant Pseudomonas aeruginosa from cancer patients. These genes play the most significant role in multi drug resistance. In this study, we used the disc diffusion method for the determination of antibiotic resistance against six antibiotics and PCR technique for the detection of genes. The results showed that the P. aeruginosa isolates were resistant against Meropenem (MEM) 68%, Imipenem (IMP) 92%, Polymyxin B (PB) 64%, Gentamicin (CN) 60%, Tobramycin (TOB) 16% and Tazobactam (TZP) 12%. The PCR results showed that 82% isolates were positive for the VIM and 72% was positive for IMP genes. These results revealed high emergence of resistance in carbapenem of P. aeruginosa isolates studied. This is the first report showing detection of carbapenem resistant gene against P. aeruginosa in cancer patient from Pakistan. The study might be helpful in understanding the molecular basis of antibiotic resistance in P. aeruginosa from cancer patients.

Key words: METALLO-BETA, PSEUDOMONAS AERUGINOSA, CANCER


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