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Determination of plasma Reactive Oxygen Species in young male albino rats after treatment with Gentamicin and Renadyl


The International Journal of Global Sciences (TIJOGS)

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Kanwal Zahra1, Sohaib Aslam2, Hamza Nazeer1, Umair Rasool1, Muhammad Umair3, Ghazia Zahid4, Arslan4

1Institute of Pharmacy, Physiology and Pharmacology, University of Agriculture Faisalabad, Pakistan

2Department of Theriogenology, Faculty of Veterinary Sciences, University of Agriculture Faisalabad, Pakistan

3Department of Parasitology, University of Agriculture Faisalabad, Pakistan

4Center of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan.

*Corresponding author’s email: zahraawan75@gmail.com
Submitted Accepted Published
May 13,2019 Aug 28,2019 Sep 02,2019

2019 / Vol: 2 / Issue: 3


Abstract


Kidney is important organ for controlling blood pressure and hypertension due to elevated level of salts. Gentamicin is aminoglycoside which is used for treatment of gram negative bacterial infection. Gentamicin is considered as nephrotoxic agent and cause cell death due to inflammation, free radical and renal blood flow. Renadyl is probiotic dietry supplement used in patient with chronic kidney disease. Experimental study had been conducted that Gentamicin was used for altering the kidney and Renadyl used for treatment of altered kidney. Thirty healthy male albino rats were divided into three (3) groups. Two groups were treated and one was controled. Equally divided ten (10) rats in each group. Gentamicin was given to rats intraperitoneally from 1st to 8th days for altering the kidney and 3rd group was treated by Renadyl orally from 9th to 18th days interval at regular basis for treatment. Blood sample was drawn out after administration of drug at 8th and 18th day and used for determination of total antioxidant, total oxidant status, glutathione reductase, glutathione peroxidase, catalase, arylestrase, and paraoxonase. All the required parameter was increased except total oxidant status (TOS), its level was decreased in the treated rats. The data was examined statistically by two way analysis of variance and

DMR.


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