• The International Journal of Biological Research (TIJOBR)- Published Quarterly
  • The International Journal of Global Sciences (TIJOGS) -Published Quarterly

Effect of multi-strain inoculation of PGPR on growth and production of rice under field conditions

The International Journal of Biological Research (TIJOBR)


Aqsa Liaqat1, M. Asim Raza2, Amira Athar2, Umar Nasser3, Fatima Farooq1, Uzma Rasool1, Sana Shaukat1, Ayesha Idrees1Zulnoon Haider4*,

1Department of BotanyUniversity of Agriculture, Faisalabad, Pakistan

2Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan.

3Institute of Molecular Biology & Biotechnology, University of Lahore, Pakistan

4Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan.

*Corresponding email: zulnoonhaider51214@gmail.com

Submitted Accepted Published
Aug 12,2019 Nov 13,2019 Jan 14,2020

2020 / Vol: 3 / Issue: 1


A field experiment was conducted in the research area of the Institute of Soil and Environmental Sciences (ISES), University of Agriculture Faisalabad (UAF) during the Kharif season of 2012 to evaluate the effect of multi-strain inoculation on the growth and yield of rice under field conditions. Three pre-isolated strains of PGPR i.e., one Rhizobium (LSI-29), one associative N-fixer (6K) and one endophyte strain (PsJN) were used. Rice seedlings (39 days old) of a fine rice variety Basmati-315 were transplanted into the field after inoculation by these strains individually and in all possible combinations. There were eight treatments including an un-inoculated control (root dipping into sterilized LB broth for one hour). Each treatment had three replications and randomized complete block design (RCBD) was followed for statistical analysis of the data collected. Recommended doses of NPK (120-60-60) for rice as urea, SSP and MOP respectively were applied in each experimental unit. Field was irrigated with good quality canal irrigation water and recommended plant protection measures were administered as needed. At physiological maturity, data regarding grain yield, number of tillers, plant height, fresh and dry biomass, number of spikes per penicle, number of spikelets per penicle, number of infertile spikelets per penicle, fresh and dry straw weight, 1000-grain weight and harvest index was recorded. Overall, it was observed that all the treatments improved almost all studied parameters of the crop as compared to un-inoculated control. The multi-strain inoculation caused maximum benefits to the crop followed by co-inoculation treatments and then single strain inoculation treatments. Multi-strain inoculation of LSI-29, 6K and PsJN increased plant height, penicle length, fresh biomass, number of tillers, number of spikes per penicle, number of spikelets per penicle, number of infertile spikelets per penicle, number of penicles, number of infertile penicles, grain yield, straw fresh weight, straw dry weight, dry biomass weight, 1000 grains weight and harvest index. The comparison of all the treatments has shown that among co-inoculation treatments, co-inoculation of PsJN and 6K is more efficient followed by co-inoculation of PsJN and LSI-29 and co-inoculation of LSI-29 and 6K, and among single strain inoculation, PsJN is more efficient than sole inoculation of 6K and LSI-29.


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