The International Journal of Biological Research (TIJOBR)
*Ghulam Zahara Jahangir1, Durre Shahwar2, Madeha Sadiq1 , Idrees Ahmad Nasir3, Habiba
Khalil4, Muhammad Iqbal1
1 Centre for Applied Molecular Biology (CAMB), University of the Punjab,Lahore-53700, Pakistan.
2 Department of Chemistry, GC University, Lahore.
3 National Centre of Excellence in Molecular Biology, University of the Punjab,Lahore-53700, Pakistan.
4 Department of Zoology, Minhaj University, Lahore, Pakistan.
*For correspondence: Email firstname.lastname@example.org
|Sep 22,2019||Dec 10,2019||Jan 13,2020|
2020 / Vol: 3 / Issue: 2
Essential oils of six spice plants namely Piper nigrum, Nigella sativa, Cinnamomum zeylanicum, Syzygium aromaticum, Cuminum cyminum and Ptychotis ajowan were evaluated for their protective properties against chemical mutagen (hydrogen peroxide). The experiments were performed on fresh human cheek cells, in vitro, using single cell gel electrophoresis/comet assay technique. Hydrogen peroxide (H2O2) was used in concentrations of 25, 50, 100, 150, 200, 250, 300, 350 and 900 µM respectively to get decipherable damage in DNA of cheek cells. Conspicuous DNA damage (comparatively larger tail lengths) was observed in human cheek cells on application of H2O2 (350 µM). The effectiveness of essential oil against H2O2 (350 µM ) was tested by evaluating the inhibitory effect of essential oils against chemical mutagen on human cheek cells . All the six essential oils were found evenly effective in shielding the DNA damage caused by H2O2 (350 µM). Furthermore the comet assay technique was found to be sternly potent contrivance to appraise the DNA damage of individual cells.
Single Cell Gel Electrophoresis/Comet Assay, Anti mutagenic Effect, DNA Damage, Cheek Cells, Essential Oils, Hydrogen Peroxide.
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