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Extraction and utilization of pomegranate seed oil in cookies to alleviate hyperlipidemia in rats


The International Journal of Biological Research (TIJOBR)

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Muhammad Fahad Latif1*, Muhammad Tayyab Aleem2, Murad Bakhsh3, Asad Sohail1, Talha Riaz1, Ahmad Bilal4

*Corresponding author:  fahadlatif77@gmail.com

Submitted Accepted Published
Oct 10,2018 Feb 21,2019 Mar 06,2019

2019 / Vol: 2 / Issue: 1


Abstract


Certain oils have some therapeutic implications which make them superior over other lipids. Pomegranate seed oil is one of these oils having potent health effects such as inhibiting carcinogenesis, improving skin tone, lipid profile and gastrointestinal health. In our study pomegranate seed oil was utilized in cookies for its efficacy against hyperlipidemia. Cookies were prepared with 15% pomegranate seed oil and these cookies were supplemented in rats chow. A total of 60 rats were randomly divided into four groups after acclimatization period of one week. Negative control group (n=15) was fed on normal diet, while hyperlipidemia was induced in positive control (G1) and fed on high fat diet throughout the study. Other two experimental groups (G2 and G3) also fed on high fat diet were having 15 rats in each group. Group G2 and G3 with hyperlipidemia were fed on high fat diet alongside 7 and 14% cookies supplemented in diet, respectively. In that 45 days study serum analyses for different biochemical parameters were performed at intervals of 0, 15, 30 and 45 days. Serum total cholesterol elucidated sharp fall in its level in G2 and G3 as compared to G1, while triglycerides levels decreased in G2 and G3 in comparison to positive control group. Serum HDL-cholesterol also elevated significantly in response to pomegranate seed oil supplemented cookies in G2 and G3 while LDL-cholesterol decreased. Lipid profile in experimental groups improved significantly in response to the dietary intervention of adding PSO supplemented cookies in chow. For total cholesterol group G0 elucidated normal range of cholesterol as it was fed on normal diet. Total cholesterol remained elevated in positive control as it was solely given high fat diet only but both the pomegranate groups (G2 and G3) showed decrease in total cholesterol. But for serum triglycerides the results shown by PSO were promising as serum triglycerides diminished in response to pomegranate seed oil supplemented cookies in both experimental groups of G2 and G3. For LDL and HDL-cholesterol PSO again remained at forefront to bring about their levels in normal range from deranged levels due to high fat diet induced dyslipidemia. HDL-cholesterol which was suppressed in response to dyslipidemia raised back to normal level of 43±1.0 mg/dl after 45 days in G2 while this increase was more evident in G3 where its level raised to 47±2.0 mg/dl from 26±2.4 mg/dl at the end of trial. For elevated LDL, PSO acted as a key suppressant as it rolled back LDL in G2 from 86±6 mg/dl to 63±2 mg/dl in group G2 and in group G3 LDL was suppressed back to normal range of 49±2 mg/dl from 87±2 mg/dl after 45 days dietary intervention.


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