• The International Journal of Biological Research (TIJOBR)- Published Quarterly
  • The International Journal of Global Sciences (TIJOGS) -Published Quarterly

Genetic studies and heritability estimates of yield contributing traits of Brassica napus L.

The International Journal of Biological Research (TIJOBR)


Mazhar Ullah Bashir1*, Rabail Afzal1, Muhammad Rizwan Gulzar1, Ahtsham Bilal1, Sana Zia1, Ifrah Amjad1, Azka Muneer1, Aqib Manzoor1and Muhammad Kamran1

-Corresponding author: aamizg167@gmail.com
Submitted Accepted Published
Feb 02,2018 Sep 10,2018 Dec 20,2018

2018 / Vol: 1 / Issue: 1


The present research was conducted to find out genetic basis and heritability estimates of yield contributing traits of Brassica napus L. Seven lines ZM-R-10, OSCAR, ZM-R-30, ZM-R-16, ZM-R-17, ZM-R-5, ZM-R-21 and three testers Hyola-401 and Winer of Brassica napus were crossed in Line x Tester scheme. Twenty-one crosses and their parents were evaluated in field by following randomized complete block design with three replications. The seed of the germplasm was sown in field maintaining row to row and plant to plant distance 75 and 25 cm, respectively. Experiment was performed during the crop season of 2016-17 and 2017-18. Data were recorded on quantitative traits i.e. plant height, number of silique per plant, silique length, number of seeds per siliqua, 1000 seed weight and seed yield per plant. The recorded data were subjected to analysis of variance. Data for the traits showing significant genotypic difference were subjected to Line x Tester analysis for estimation of combining ability effects. Heritability and heterosis was estimated by statistical technique. Analysis of variance showed significant differences for all the traits in crosses parent, lines, and testers. It indicated the existence of considerable genetic variability in breeding material. Plant height ranged from 251cm to 142cm, number of silique per plant from 235cm to 61cm, silique length front 7.02cm to 5.31cm, number of seeds per silique 30 to 16, seed weight from 7.04g to 4.04g and seed yield per plant from 27.68g to 10.13g. Among lines ZM-R-17, ZM-R-30 and ZM-R-21 and testers Hyola-401 and Winer had positive and significant general combining ability for most of the traits. ZM-R-30 × Hyola-401, ZM-R-16 × Hyola-401, ZM-R-5 × Hyola-401 and OSCAR × Hyola-401 while ZM-R-5 × Winer had highest means for most of the yield related traits. OSCAR × Winer, ZM-R-16 × Hyola-401, ZM-R-5 × Glarchi, ZM-R-5 × Hyola-401 and ZM-R-21 × Glarchi had positive and significant specific combining ability. ZM-R-5 × Winer, ZM-R-30 × Glarchi and ZM-R-30 × Hyola-401 had maximum heterosis for yield and its related traits. The variances due to specific combining ability (SCA) was more than general combining ability (GCA) which showed the role of non-additive gene action for these traits. Narrow sense heritability estimates ranged from 0.01 to 0.45. Plant height, silique length, and 1000 seed weight had low heritability. Number of siliquae per plant, number of seeds per silique showed medium heritability. Seed yield per plant, number of primary branches per plant showed high heritability among all the traits. Phenological traits were also showed variation in growth habit, leaf shape, siliqua shape, siliqua colour and seed colors.

Key words: Heritability, combining ability, Brassica napus L.


  1. Abideen, S.N.U., F. Nadeem and S.A. Abideen. 2013. Genetic variability and correlation studies in (Brassica napus L.) genotypes. Int. J. Appl. Studies 2: 574-581.
  2. Ahsan, M.Z., F.A. Khan, S.A. Kang and K. Rasheed. 2013. Combining Ability and Heterosis Analysis for Seed Yield and Yield Components in Brassica napus L. J. Biol. Agri. Health. 3: 31-36.
  3. Akbar, M., B. Tahira, H.M. Atta and M. Hussain. 2008. Combining ability studies in rapeseed (Brassica napus L.). Int. J. Agri. Biol. 10(2):205-208.
  4. Akbar, M., B. Tahira, H.M. Atta and M. Hussain. 2008. Combining ability studies in rapeseed (Brassica napus L.). Int. J. Agri. Biol. 10(2):205-208.
  5. Aytac, Z. and G. Kinaci. 2009. Genetic variability and association studies of some quantitative characters in winter rapeseed (Brassica napus L.). African J. Biotechnol. 8 (15):3547-3554.
  6. Azizinia, S. 2012. Combining ability analysis of yield component parameters in winter rapeseed genotypes (Brassica napus L.). J. Agri. Sci. 4(4):87-94.
  7. Dar, Z.A., S.A. Wani, Z. Gul, M. Habib and I. Ahmad. 2011. Combining ability analysis in brown sarson (Brassica campestris L.) under Kashimir conditions. Appl. Biol. Res. 13: 74-77.
  8. Farshadfar, E., Z. Kazemi and A. Yaghotipoor. 2013. Estimation of combining ability and gene action for agro-morphological characters of rapeseed (Brassica napus L.) using line× tester mating design. Int. J. Adv. Biol. Biomed. Res. 1(7): 711-717.
  9. Meena, H., A. Kumar, B. Ram, V. Singh, P. Meena, B. Singh and D. Singh. 2015. Combining ability and heterosis for seed yield and its components in Indian mustard (Brassica juncea L.). J. Agri. Sci. Technol. 17: 1861.1871
  10. Muhammad, A., Raziuddin, M. Sajid, Q.U. Bacha, A. Rahman and S.A. Khan. 2014.  Combining ability and heritability studies for yield contributing traits in F2 populations of Brassica napus.  American-Eurasian J. Agri. Environ. Sci. 14: 509-515.
  11. Nasim, A., N.U.K. Farhatullah, N.U. Khan, M. Afzal, S.M. Azam, Z. Nasim and N. Amin. 2014. Combining ability and heterosis for yield and yield contributing traits in Brassica rapa (L.) ssp. dichotoma (roxb.) hanelt. Pak. J. Bot. 46:2135-2142.
  12. Rameeh, V. 2012. Combining ability analysis of plant height and yield components in spring type of rapeseed varieties (Brassica napus L.) using line × tester analysis. Int. J. Agri. For. 2(1): 58-62.
  13. Saeed, F., M.H.N. Tahir, S.A. Kang, M. Riaz, J. Farooq and M. Saeed. 2013. Heterosis and combining ability for seed yield and its components in Brassica juncea L. Albanian J. Agric. Sci. 12: 203 -208.
  14. Shaukat, S., F.U.K. Raziuddin and I.A. Khalil.2014. Genetic variation and heritability estimates of quality traits in Brassica napus L. J. Biol. Agri. Healthcare 20(4):1-4.
  15. Sincik, M., A.T. Goksoy and Z.M. Turan. 2011. The heterosis and combining ability of diallel crosses of rapeseed inbred lines. Not. Bot. Hort. Agro. 39: 242-248.
  16. Singh, A., R. Avtar, D. Singh, O. Sangwan, N. Thakral, V. Malik, B. Goyat and U. Dalal. 2013. Combining ability analysis for seed yield and component traits in Indian mustard (Brassica juncea L.). Czem and Coss. J. Res. Plant Biol. 3(2): 26.31.
  17. Tuncturk, M. and V. Ciftci, 2007. Relationships between yield and some yield components in rapeseed (brassica napus ssp. Oleifera l.) cultivars by using correlation and path analysis. Pak. J. Bot. 39(1): 81
  18. Turi, N.A., Raziuddin, Farhatullah, N.U. Khan, G. Hassan, J. Bakht, S. Khan and M. Shafi. 2011. Combining ability for yield related traits in Brassica juncea. Pakistan J. Bot. 43(2): 1241-1248.

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