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Isolation, purification and management of Xanthomonas oryzae pv. oryzae causing bacterial leaf blight disease in rice


The International Journal of Biological Research (TIJOBR)

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Sana Rubab1*, Naeem Ullah1, Faiz-ul-Hassan1, Zulnoon Haider2, Muhammad Athar shafique1, Rabia Zafar1

Hamza Aslam1, Bushra Rehman1, Ayesha Idrees1

1Department of Botany, University of Agriculture, Faisalabad, Pakistan.

2Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan.

*Corresponding author’s: rubhashmi@gmail.com 
Submitted Accepted Published
Sep 26,2019 Nov 10,2019 Jan 10,2020

2020 / Vol: 3 / Issue: 1


Abstract


Rice is an important crop of Pakistan and majority of people depends on this because this is either their food or source of earning. Rice is staple food after wheat in Pakistan. The production of rice has been always more than the domestic needs, so every year Pakistan earns a lot of credit due to exports of rice. Rice is a cereal consumed by majority of people worldwide due to its high digestibility and fragrance. Worldwide its third important cereal on the basis of production after wheat and maize and regarding nutritional value it is 5th important food which is rich in all important nutritional components. Rice provides 20 percent of the world’s dietary supply. There is decline in the rice production due to its vulnerability towards microbial diseases and abiotic agents. Rice crop is vulnerable to disease like root rot, wilt, foot rot, nematode invasions, and bacterial blight (BLB) of rice. BLB of rice is one of the oldest diseases and attacks almost all parts of the plant primarily the leaves. In this study, disease sample were collected from District Hafizabad, which is an important area famous for rice cultivation since centuries. The rice diseased sample were brought to the lab of department of plant pathology. The samples were collected during the optimum season of the disease and pathogen was isolated on the nutrient agar media. The pathogen was identified as Xanthomonas oryzae pv. oryzae (Xoo) based on color, shape of colony, growth rate, appearance under UV light and biochemical tests. From the available sample four isolates were chosen for in-vitro test. These four bacterial isolates were tested against four antibiotics in the 96 well plates. Different concentrations of antibiotics were used to the most efficient inhibitory dose. Meanwhile in the field, 12 varieties were sown in the field and these varieties were given the inoculum pressure of Xoo. Most of the genotypes of rice were observed as susceptible to this bacterial pathogen and no one was immune. There was a mix response of the genotypes of rice to the pathogen.

Key words: Rice, Bacterial leaf blight, antibiotics


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