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Polyphasic Taxonomy of Fusarium causing wilt in cut flower crops (Chrysanthemum and Marigold) and its chemical management


The International Journal of Biological Research (TIJOBR)

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Umar Muaz1*, Arooba Nawaz2, Akasha Mansoor2, Amar Ahmad Khan1, Zulnoon Haider1, Kamran Ahmad2

1Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan

2Department of Botany, University of Agriculture, Faisalabad, Pakistan.

*Corresponding author email: umarmuaz175@gmail.com

Submitted Accepted Published
Jan 09,2020 Feb 26,2020 Mar 15,2020

2020 / Vol: 3 / Issue: 3


Abstract


Marigold (Tagetes erecta L.)  and Chrysanthemum (Chrysanthemum L.) are important cut flower crops which are facing threat by wilting disease in Pakistan. Survey of important ornamental plant local nurseries, public parks and gardens of districts of Punjab Faisalabad, Lahore, Kasur, Vehari, and Islamabad were done. Samples of soil, root, shoot and leave from healthy as well as wilted portion of both crops were collected. Isolation was done to find the Fusarium species associated with diseased samples. Fusarium spp. was characterized using morphological characters. Cultural characters of Fusarium spp. on potato dextrose agar medium (color, texture and growth pattern) were studied. Microscopic characters of Fusarium equiseti on different magnification (Mycelial structure, conidia shape and size) were observed. Molecular characterization of morphologically identified Fusarium equiseti was done and submitted to Gene bank with accession no. MN135748 and MN135746. Characterized Fusarium equiseti was preserved on agar slants and dry filters papers in FMB-CC-UAF with Accession No. FMB0151, FMB0152. Pathogenicity was confirmed following by Koch’s postulates. Chemical control is one of the best management strategies that is used commonly to control the diseases. Different fungicides Score, Limin Lingx, Notyl and Myco Guard were used at different concentration 100, 200, 300 ppm in in-vitro conditions against pathogenic Fusarium equiseti. Percent inhibition of Limin Lingx was observed 89%. Most effective fungicide Limin Lingx was applied on diseased plants and after four weeks disease severity was reduced up to 60%. 

Key words: cut flower, Chrysanthemum, Marigold, chemical management


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